This system, bimolecular fluorescence complementation, allows one to visualize protein-protein interactions in living plant cells. Finally, we have used microarray and bioinformatic analyses to identify plant genes that respond to Agrobacterium infection.
Methods in Molecular Biology Agrobacterium Protocols Agrobacterium Protocols. Volume II. Editors: Wang, Kan (Ed.) Free Preview Pages Song. Agrobacterium Protocols offers beginning and experienced researchers the most Part of the Methods in Molecular Biology™ book series (MIMB, volume 44).
Many of these genes are involved in host defense responses. Hwang, H. Plant Sci. Wei, F. Plant J. DOI: Altpeter, F.
Plant Cell Doi: Iwakawa, H. Plant-Microbe Interact. Gelvin, S. Lin, C.
Plant Biotechnol. In press. Tao, Y. Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2. USA Gaspar, Y. Characterization of the Arabidopsis lysine-rich arabinogalactan-protein AtAGP17 mutant rat1 that results in a decreased efficiency of Agrobacterium transformation.
Plant Physiol. Lee, L. Osa protein constitutes a strong oncogenic suppression system that can block vir-dependent transfer of IncQ plasmids between Agrobacterium cells, and the transfer of T-DNA and IncQ plasmids to plant cells. Plant proteins that interact with VirB2, the Agrobacterium pilin protein, are required for plant transformation. Viral-mediated plant transformation gets a boost News and Views. Nature Biotechnol. Yi, H.
Citovsky, V. Subcellular localization of interacting proteins by bimolecular fluorescence complementation in planta.
Transgenic Arabidopsis plants expressing Agrobacterium tumefaciens VirD2 protein are less susceptible to Agrobacterium transformation. Plant Pathol. Agrobacterium virulence gene induction. In Methods in Molecular Biology: Agrobacterium protocols. Wang, ed. Humana Press, Totowa, NJ. Agrobacterium transformation of Arabidopsis thaliana roots: A quantitative assay. Using BY-2 cells to investigate Agrobacterium-plant interactions.
Nagata, K. Matsuoka, and D. Springer Berlin, Heidelberg, New York. Acta Kim, S. Genome-wide analysis of Agrobacterium T-DNA integration sites in the Arabidopsis genome generated under non-selective conditions. Crane, Y. RNAi-mediated gene silencing reveals involvement of Arabidopsis chromatin genes in Agrobacterium-mediated transformation.
Novel plant transformation vectors containing the super-promoter. Function of host proteins in the Agrobacterium-mediated plant transformation process. In Agrobacterium, from biology to biotechnology T. Tzfira and V.
Citovsky, eds. Springer, N. T-DNA binary vectors and systems.
Agrobacterium-mediated DNA transfer, and then some. News and Views Bhattacharjee, S. Cao, H. Vectors for multi-color bimolecular fluorescence complementation to investigate protein-protein interactions in living plant cells. Growth and Storage of Agrobacterium.
Pages Jones, Andrew L. Agrobacterium Virulence. Agrobacterium tumefaciens Chemotaxis Protocols. Binary Ti Plasmid Vectors. Leaf Disk Transformation. Ian S. Curtis, Michael R. Davey, J.
Brian Power. Peanut Transformation. Agrobacterium -Mediated Transformation of Potato Genotypes. Julie Graham, Ronnie J.
McNicol, Amar Kumar. Transformation Protocols for Broadleaved Trees. Trevor M. Fenning, Kevan M. Michael R. Davey, Rajendra S. Patil, Kenneth C. Lowe, John B. Histochemical GUS Analysis. Phillips, Kevan M. Kevan M.